Laboratory Consumables in Proteomics
It is a rapidly expanding field of molecular biology concerned with the systematic, high-throughput analysis of a cell's or organism's protein expression. Proteomics is the study of proteomes on a large scale. A proteome is a collection of proteins made by a living organism, system, or biological context. We can talk about a species' proteome (for example, Homo sapiens) or an organ's proteome (for example, the liver). The proteome is dynamic, varying from cell to cell and changing over time. The proteome reflects the underlying transcriptome to some extent. However, in addition to the expression level of the relevant gene, many other factors influence protein activity (which is often measured by the reaction rate of the processes in which the protein is involved).
ELISA is a plate-based assay technique for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The activity of the reporter enzyme is measured by incubating it with the appropriate substrate to produce a measurable product, which is then measured. A highly specific antibody-antigen interaction is the most important aspect of an ELISA.