How to pipette properly. For an air displacement pipette, follow these steps below will increase your pipetting accuracy during the process.
Set the right volume you want to use, insert the pipette into a tip with light pressure, don't be too hard or you will damage the pipette or the tip.
Press down the plunger botton and keep the aspiration angle at vertical, keep the immersion depth as small as possible to avoid capillarity effect causing more liquid flowing into the tip.
Slowly release the plunger botton and wait for the pipette to fully draw the liquid.
When dispensing liquid,hold the pipette at 20 to 45 degrees with contatc to the wall, and smoothly press the plunger botton down.
Repeat step3 and setp4 2-3 times in the same vessel to pre-wet the tip before a formal liquid transition.
After finish the process, press ejection botton to remove the tip and place the pipette back to the shelf.