Relevant Classification and Scope of Use of Deep Well Plates
On the basis of the appearance size of the ordinary microplate, the deep well plate keeps the length and width in line with the SBS specification, and at the same time increases the depth of the hole, so as to achieve the purpose of increasing the volume of each hole. And in order to adapt to its specific range of use, on the one hand, by changing the manufacturing materials (polypropylene (PP) is currently used, and polystyrene (PS) is used individually), on the other hand, by improving the surface treatment process, a class of laboratory plates made from it.
Ⅰ. Classification of deep well plates:
1. According to the number of holes, the more common ones can be divided into 96-well microplates and 384-well plates.
2. According to the hole type, the 96-well plate can be mainly divided into round hole type and square hole type. The 384-well plates are all square wells.
3. According to the shape of the bottom of the hole, there are two common types: U-shaped and V-shaped.
Ⅱ. Application scope of deep well plate:
1. Storage samples:
It can replace the conventional centrifuge tube to store samples, and it can be placed neatly during storage, saving space, and has a large storage capacity, which can withstand -80 ℃ refrigerator. Therefore, it is also called a storage block.
2. Processing samples:
It can be combined with a discharge gun, high-throughput automatic liquid manipulation instruments and software to achieve high-throughput operations on biological samples, such as protein precipitation and liquid-liquid extraction. Greatly improve the efficiency of sample processing. PP material can withstand 121 ℃ high temperature and high pressure sterilization.
3. Sampling operation:
Commonly used in various autosamplers, it can be directly placed in the sample chamber of the autosampler for injection. Compared with the traditional sample injection vial, it can not only double the number of samples in the sample chamber, but also realize the sample storage in the sample chamber. The 96-well plate can be directly injected after processing, eliminating the tedious work of drawing samples back and forth, placing samples, covering lids, inserting cannulas, and washing bottles.